Figure 6
From: Structure based analysis of KATP channel with a DEND syndrome mutation in murine skeletal muscle
Effects of glucose uptake in Kir6.2/R50P-expressing myotubes. (a–c) Confocal laser-scanning microscope images of Kir6.2/WT-expressing myotubes. 2-NBDG fluorescence (a), Kir6.2/WT localization (b), merged image of (a,b) (c). (d–f) Confocal laser-scanning microscope images of Kir6.2/R50P-expressing myotubes. 2-NBDG fluorescence (d), Kir6.2/R50P localization (e), merged image of (d,e) (f). Scale bars 100 μm. (g) Mean value of 2-NBDG uptake between non-transfected, Kir6.2/WT, Kir6.2/R50P-expressing myotubes. (h) pAAV vector design, the Kir6.2-expresing myotubes show red fluorescence. The intensities were measured using ImageJ software (Version 1.51; https://imagej.nih.gov/ij/download.html), and the figures were analyzed and created by GraphPad Prism software (GraphPad Prism 8.2.1).
