Figure 4

Average proportions of locus dropout by locus for single cells at 29, 30 and 31 cycles using the PowerPlex Fusion 6c kit analyzed at (a) 10 RFU, (b) 100RFU and (c) 150RFU. Cycle numbers within each locus from left to right are 29, 30, and 31, respectively and the colors (blue, green, yellow, red and purple) represent the dye channel.