Table 2 Summary of the mean cycle set allele and locus dropout, the range (minimum-to maximum values observed separated by cycle number and the analytical threshold value used to assess the signal) and the p values of the ANOVA comparing the variance at each threshold across cycle numbers at α = 0.99.
Threshold (RFU) | Cycle # | n | Proportion samples w/full dropout | Allele dropout | Locus dropout | ||||
|---|---|---|---|---|---|---|---|---|---|
Average | Range | p value | Average | Range | p value | ||||
10 | 29 | 38 | 0/38 | 0.405 ± 0.223 | 0.044–0.791 | 0.688 | 0.257 ± 0.194 | 0.00–0.667 | 0.458 |
30 | 22 | 0/22 | 0.369 ± 0.199 | 0.022–0.744 | 0.233 ± 0.191 | 0.00–0.667 | |||
31 | 21 | 0/21 | 0.429 ± 0.269 | 0.044–0.954 | 0.313 ± 0.277 | 0.00–0.917 | |||
100 | 29 | 38 | 1/38 | 0.675 ± 0.280 | 0.154–1.00 | 0.129 | 0.579 ± 0.320 | 0.00–1.00 | 0.111 |
30 | 22 | 0/22 | 0.550 ± 0.289 | 0.044–0.977 | 0.429 ± 0.309 | 0.044–0.958 | |||
31 | 21 | 0/21 | 0.538 ± 0.294 | 0.044–0.977 | 0.430 ± 0.311 | 0.044–0.958 | |||
150 | 29 | 38 | 7/38 | 0.788 ± 0.234 | 0.154–1.00 | 0.0096 | 0.712 ± 0.287 | 0.00–1.00 | 0.0073 |
30 | 22 | 0/22 | 0.619 ± 0.299 | 0.044–0.977 | 0.508 ± 0.330 | 0.00–0.958 | |||
31 | 21 | 1/21 | 0.581 ± 0.300 | 0.044–1.00 | 0.474 ± 0.322 | 0.00–1.00 | |||
