Table 3 Thermodynamic parameters associated with the heat-induced denaturation of WT cyt c and its L94G mutant in the native buffer (pH 6.0).a,b.

From: Stability of uniformly labeled (13C and 15N) cytochrome c and its L94G mutant

Proteins

Thermodynamic parameters

Tm, °C

∆Hm, kcal mol−1

∆Cpc, kcal mol-1 K−1

∆GD0, kcal mol−1

WT cyt c (unlabeled)

81.80 ± 0.40

(80.40 ± 0.20)

98 ± 2

(97 ± 2)

1.22 ± 0.07

(1.22 ± 0.07)

9.81 ± 0.18

(9.48 ± 0.19)

WT cyt c (labeled)

82.50 ± 0.34

98 ± 2

1.22 ± 0.07

9.84 ± 0.15

L94G mutant (unlabeled)

55.00 ± 0.20

(54.10 ± 0.30)

72 ± 3

(72 ± 2)

0.70 ± 0.2

(0.70 ± 0.2)

5.56 ± 0.14

(5.6 ± 0.2)

L94Gmutant (labeled)

54.40 ± 0.10

72 ± 3

0.70 ± 0.2

5.48 ± 0.13

  1. a± represents the deviation from the mean value of triplicate measurements.
  2. bIn the presence of 30 mM sodium cacodylate and 100 mM NaCl (pH 6.0).
  3. cValues taken from50,51. Values in parenthesis are from DSC measurements.
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