Figure 4 | Scientific Reports

Figure 4

From: The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures

Figure 4

Bacterial colonization of C. limon seeds. Confocal laser scanning microscopy images showing Fluorescence In Situ Hybridization (FISH)-stained endophytic bacteria of Citrus limon seed cryosections. First and second row show the result after staining with the Rhodamine red-labelled universal FISH-probes EUB338MIX (shown as red in panel (B) and (F), circles) and the Cy5-labelled Firmicutes-specific probe LGC354MIX (shown as green in panel G, circles); panel (D) and (H) are the overlaps of the images (B, C) (cells stained by the universal probe EUB338MIX only) and (F–G), respectively (circle in the panel H indicates Firmicutes cells); third, fourth and fifth row show the result after staining with the Rhodamine red-labelled universal FISH-probes EUB338MIX (shown as red in panel (J), (N) and (R), arrows) and the Cy5-labelled Gammaproteobacteria–specific probe GAM42a (shown as green in panel K and O, arrows); panel (L), (P) and (T) are the overlap of the images (I–K), (M–O) and (Q–S) respectively (arrows in the panels (L) and (P) indicate Gammaproteobacteria cells, while bidirectional arrow in T highlights bacteria cells stained by the universal probe EUB338MIX only); first column panels (A, E, I, M and Q) show the autofluorescence of the seed tissues. Scale bars: 20 μm (all panels). pa parenchyma, tg tegument or seed coat (mesotesta), vt vascular tissues, b bacteria, g Gammaproteobacteria.

Back to article page