Table 2 Results PCR screening. Bats were screened for the presence of virus nucleic acids by different PCR assays.

From: The virome of German bats: comparing virus discovery approaches

PCR assays

Assay type

Bats

#/novel/size*

Assay reference

Virus reference

Adenoviridae

Adenoviridae (Polymerase)

Pan

79/14/203H

(67)

(31)

Arenaviridae

Old-World

Pan

0/0/60

(61)

Unpublished

Bunyaviridae

Hanta Virus Puumala

cPCR

0/0/150

In-house design RKI

(71)

Hanta Virus Dobrava

cPCR

0/0/150

In-house design RKI

(71)

Hanta Virus Tula

cPCR

0/0/150

In-house design RKI

(71)

Hantaviruses

Pan

0/0/180

(64)

Unpublished

Nairoviruses

Pan

0/0/150

(65)

Unpublished

Phleboviruses

Pan

0/0/150

(65)

Unpublished

Coronaviridae

Coronaviridae

Pan

1/1/240

(35)

Unpublished (MN851285)

Coronaviridae

Pan

0/0/90

In-house design RKI

unpublished

Flaviviridae

Flaviviridae

Pan

0/0/150

In-house design

Unpublished

Flaviviridae

Pan

0/0/180

(63)

(71)

Herpesviridae

BatGHV1

cPCR

1/1/180

(34)

(71)

BatGHV3

cPCR

7/1/180

(34)

(71)

BatGHV4

cPCR

22/1/210

(34)

(71)

BatGHV5

cPCR

11/1/210

(34)

(71)

BatGHV6

cPCR

24/1/210

(34)

(71)

BatGHV7

cPCR

2/1/210

(34)

(71)

BatBHV1

cPCR

1/1/180

(34)

(71)

Herpesviridae

pan

11/9/60

(68)

Unpublished

Orthomyxoviridae

Influenza A

qPCR

0/0/330

(66)

Paramyxoviridae

Res-Mor-Hen

Pan

2/2/120

(60)

(32)

PAR

Pan

3/3/180

(60)

(32)

Poxviridae

Low GC poxviruses

Pan

0/0/263

(70)

Unpublished

  1. pan generic family-specific assay, qPCR quantitative real-time PCR, cPCR conventional specific PCR. *#/novel/size: #, number of positive samples; novel, number of novel viruses obtained; size, bat sample size. HScreened in collaboration with our Hungarian colleagues (see Ref.33).