Figure 4

Variation of surface expression of erythrocyte receptors between donors. Dot plots of relative median fluorescent intensity (Y-axis) associated with fluorescently labelled antibodies against specific erythrocyte receptors (GYPAB, GYPA, GYPC, CR1 and DAF) from 20 blood donors (X-axis). Data were acquired by a BD LSR Fortessa X-20 flow cytometer and processed with FlowJo v.10.01. The data were stratified following normalization and donors were classified as high (Mean + 2SD, upper line), medium (between Mean ± SD, yellow area) and low expressers (Mean-2SD, bottom line). Graphs were plotted using GraphPad Prism v.8.01. Data normalization was performed as follow MFIs as follow \(CorrMFIS = MFIS*\frac{MFIMean}{{MFIC}}\), where ‘‘CorrMFI’’ is the corrected mean fluorescence intensity of a given donor, ‘‘MFIS’’ is the uncorrected mean fluorescence intensity of the sample, ‘‘MFICMean’’ is the mean mean fluorescence intensity of all the control samples, and ‘‘MFIC’’ is the mean fluorescence intensity of the control for that sample.