Figure 5
From: Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
Correlation of erythrocyte receptor densities with the efficiency of enzyme treatment. The density of erythrocyte surface receptors, labelled with specific monoclonal antibodies prior to, and following enzyme treatment was quantified by flow cytometry using fluorescently labelled secondary antibodies. The efficiency of enzyme treatment was then expressed as follows: 100 – (MFITreated Erythrocytes/MFIControl Erythrocytes × 100). Experiments were performed in triplicates and the resulting data were analysed with FlowJo v.10.01 and the Spearman R correlation were performed using Graph Pad Prism v.8.01.
