Figure 1
From: Extracellular vesicles are associated with C-reactive protein in sepsis
Characterization of plasma samples from sepsis patients and healthy individuals. (a) Plasma from sepsis patients and (b) plasma from healthy individuals was stained with anti-CRP-FITC and Anx5-PE to identify CRP-carrying EVs (CRP+ EVs) using flow cytometry. Detergent lysis with Triton X-100 abolished the EV cloud, confirming the presence of intact vesicles. Total EV counts (c), and the association of EVs with CRP (d) were determined in plasma from sepsis patients (n = 30) as compared to healthy individuals (n = 5); (e) Levels of plasma CRP in sepsis patients vs. healthy individuals. Data are given as mean ± SD and were compared using a Mann–Whitney test; *p < 0.05, ***p < 0.001. (f) EVs were pelleted from plasma by centrifugation and both EV-depleted supernatant and the EV pellet (15 µg protein each) were separated by SDS-PAGE under reducing conditions and probed for CRP by Western blotting. Human CRP was used as positive control (0.5, 0.1, 0.05 µg).
