Table 1 Fluorochrome-labeled antibodies used for flow cytometry and Western blotting.
From: Extracellular vesicles are associated with C-reactive protein in sepsis
Antigen | Source | Clone | Marker for | Fluorochrome | Abbreviation | Dilution | Supplier | Catalog number |
|---|---|---|---|---|---|---|---|---|
Flow cytometry | ||||||||
CD14 | Mouse | RMO52 | Monocytes | Phycoerythrin | PE | 1:10 | Beckman Coulter | A07764 |
CD14 | Mouse | RMO52 | Monocytes | Allophycocyanin Alexa Fluor 750 | APC AF750 | 1:50 | Beckman Coulter | B92421 |
CD41 | Mouse | P2 | Platelets | Phycoerythrin Cyanin 7 | PC7 | 1:20 | Beckman Coulter | 6607115 |
CD45 | Mouse | J33 | Leukocytes | Pacific Blue | PB | 1:10 | Beckman Coulter | A74763 |
CD235 | Mouse | HIR2 (GA-R2) | Red blood cells | Allophycocyanin | APC | 1:50 | eBioscience | 17–9987-42 |
CRP | Goat | Not applicable | C-reactive protein | Fluorescein Isothiocyanate | FITC | 1:100 | Abcam | ab34659 |
Annexin5 | Not applicable | Not applicable | Phosphatidylserine | Phycoerythrin | PE | 1:40 | BD Biosciences | 556421 |
Western blotting | ||||||||
CRP | Mouse | CRP-8 | C-reactive protein | – | – | 1:30,400 | Sigma Aldrich | C1688 |
