Figure 2 | Scientific Reports

Figure 2

From: Sensitivity of cells to ATR and CHK1 inhibitors requires hyperactivation of CDK2 rather than endogenous replication stress or ATM dysfunction

Figure 2

Induction of γH2AX by MK-8776 and AZD6738 in U2OS and AsPC-1 cells. (A) U2OS cells were incubated with the indicated concentrations of MK-8776 (upper panel) or AZD6738 (lower panel) for 6 h (not shown) or 24 h, then fixed and analyzed by flow cytometry for DNA content, γH2AX and pHH3. The percentage of cells positive for γH2AX or pHH3 is shown, and the results for γH2AX-positive cells are graphed. (B) ASPC-1 cells were incubated with MK-8776 and AZD6738 and analyzed by flow cytometry as in A. (C) 2008 and SW620 cells were incubated with AZD6738 for 6 or 24 h and analyzed as in A. The original data for panels B and C are presented in Supplementary Fig. 1.

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