Figure 5 | Scientific Reports

Figure 5

From: Sensitivity of cells to ATR and CHK1 inhibitors requires hyperactivation of CDK2 rather than endogenous replication stress or ATM dysfunction

Figure 5

Impact of partial or complete ATM loss on S-phase arrest and sensitivity to combined SN38 and ATMi. (A) MDA-MB-231 wild-type, ATM(1), and ATM(0) cells were incubated with the indicated concentrations of SN38 for 24 h, then fixed and analyzed by flow cytometry for DNA content. (B) Cells were incubated with the 0–5 µM SN38 with or without 1 µM KU60019 (ATMi) for 24 h, drugs were removed and cells grown for an additional 6 days. Cells were fixed, DNA quantified and graphed versus SN38 concentration for each cell line. (C) Each cell line was incubated with SN38 with or without and ATMi as in B, and the GI50 concentrations assessed.

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