Figure 3 | Scientific Reports

Figure 3

From: Molecular mechanisms of esophageal epithelial regeneration following repair of surgical defects with acellular silk fibroin grafts

Figure 3

Temporal proteomic and in silico analyses of signaling pathway modulation in rat esophageal tissues following reconstruction with acellular grafts. (a) Heatmap and unbiased clustering of signaling cascades based on z-score activation over the course of esophageal healing. N = 4 animals were analyzed per experimental condition. Each column represents pathway status of NSC or neotissues at experimental timepoints. Red = activated, Green = inhibited, Grey = baseline normalized to nonsurgical controls (NSC). The heatmap was generated using pHeatmap package (v1.0.12) in RStudio (v1.3.1093) detailed at http://www.rstudio.com/. (b,c) Line charts of activation z-scores of pathways contained in Clusters 3 (b) and 4 (c). (d,e) Radar graphs of significantly activated pathways Clusters 3 (d) and 4 (e) demonstrating biological functions. (f) Word clouds showing the most recurrent proteins and their functions in pathways identified in Clusters 3 [top plot] and 4 [bottom plot]. (g,h) Heatmaps showing the level of internal connectivity (shared proteins) between pathways in Clusters 3 (G) and 4 (h).

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