Figure 2 | Scientific Reports

Figure 2

From: Systemic alterations play a dominant role in epigenetic predisposition to breast cancer in offspring of obese fathers and is transmitted to a second generation

Figure 2

Development of transplanted mammary glands in CO or OID daughters (F1). Histological depiction of transplanted mammary gland in (a) [CO(CO-M.G)], [CO(OID-M.G)], and [OID(CO-M.G)] groups. Graphs below show values for mammary gland area (b), epithelial branching (c), epithelial elongation (d) and number of terminal end buds (TEB) (e), (b–e, n = 6–13); Photomicrograph of Ki-67 immunostaining (f) (20×, staining indicated by arrows) and apoptotic cells (g) (H&E morphological assessment, 40×, cells indicated by arrows). Graphs below show proliferation index (h), number of apoptotic cells (i) and proliferation/apoptosis ratio (j), (f–i, n = 4–12). The data are expressed as mean ± SEM. Significance differences between groups were determined by one-way ANOVA followed by post-hoc analysis (mammary gland area, branching density, epithelial elongation, number of TEBs, cell proliferation and apoptosis numbers). “a” indicates statistically significant difference (P ≤ 0.05) between OID(CO-M.G) and CO(CO-M.G); “b” indicates statistically significant difference (P ≤ 0.05) between OID(CO-M.G) and CO(OID-M.G).

Back to article page