Figure 4 | Scientific Reports

Figure 4

From: Systemic alterations play a dominant role in epigenetic predisposition to breast cancer in offspring of obese fathers and is transmitted to a second generation

Figure 4

Paternal OID reprograms the sperm small non-coding RNA load in fathers (F0) and sons (F1). (a,b) Scatterplot of sperm tRNA fragments (tRF) from OID (y-axis) fathers (F0, a) and OID sons (F1, b) versus their respective controls (CO, x-axis) (n = 3–4/group) assessed by RNA-seq. (c,d) Heat-map showing differentially expressed tRNA fragments (tRFs) in sperm from OID fathers (c) and sons (d) compared to CO, highlighting overlapping tRFs in F0 and F1 (boxes). (e) Levels (fold change) of the 5 tRFs with overlapping differential expression in both OID fathers(F0) and sons (F1) compared to CO. (f) Gene ontology molecular functions significantly enriched in the targets of ValTAC, SerCGA, ArgCCG, ArgTCG and SeCTCA. Heatmaps were created using gplots (v3.1.1) package: https://cran.r-project.org/web/packages/gplots/index.html.

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