Figure 1 | Scientific Reports

Figure 1

From: An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue

Figure 1

Overview of biolistic bombardment of onion epidermis tissue using the double-barrel device. (A) A detailed view of the double-barrel in the PDS-1000 gene gun; (B) schematic diagram demonstrating the modifications to the biolistic procedure to accommodate the double-barrel device; (C) a typical tile-scan fluorescent image of onion tissue bombarded with plasmid DNA with a gene expressing GFP. The dark cross in the middle is marked to separate the sample areas impacted by the two sides of the double-barrel device.

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