Figure 1

Silencing PERK regulated RMRP expression. Huh7 and HLE cells were seeded in a 6-well flat-bottomed plate and transfected with control siRNA, PERK siRNA1, or PERK siRNA2. 24 h after treatment, RNAs and proteins were extracted and analyzed. mRNA expression of PERK was measured with RT-PCR. PERK expression was markedly suppressed by PERK siRNA. Means ± SEM for six replicates are shown. **p < 0.01 compared to control siRNA group with the Student’s t-test (A). Levels of PERK, eIF2α, and phosphorylated eIF2α were determined with Western blotting. The anti-PERK antibody, anti-eIF2α antibody and anti-phospho eIF2α antibody using in this figure were product number anti-PERK (product number: 3192), anti-eIF2α (5324), anti-phospho eIF2α (3398). PERK and phosphorylated eIF2α were suppressed by PERK siRNA. eIF2α expression was not changed between control siRNA group and PERK siRNA group. The original gel images of Western blotting are shown in Supplemental Fig. S1 (B). RNA expression of RMRP was measured with RT-PCR analysis. PERK downregulation led to increase RMRP expression. Means ± SEM for six replicates are shown. *p < 0.05 and **p < 0.01 compared to the control siRNA group with the Student’s t-test (C). Levels of RMRP were determined with Northern blotting. 28S and 18S are shown as control bands. The original gel images of Northern blotting are shown in Supplemental Fig. S2 (D).