Figure 2
PERK overexpression leads to RMRP downregulation. Huh7 and HLE cells were seeded in a 6-well flat-bottomed plate and transfected with control plasmid or PERK overexpression plasmid. 24 h after treatment, RNAs and proteins were extracted and analyzed. Fold changes in PERK mRNA expression were measured with RT-PCR. PERK expression was increased by PERK overexpression plasmid. Means ± SEM for six replicates are shown. **p < 0.01 compared to the DMSO group with the Student’s t-test (A). Levels of PERK, eIF2α, and phosphorylated eIF2α were determined with Western blotting. Phosphorylated eIF2α was increased by PERK overexpression plasmid. eIF2α expression was not changed between control group and PERK overexpression group. The original gel images of Western blotting are shown in Supplemental Fig. S3 (B). Expression of RMRP was measured with RT-PCR. RMRP expression was decreased by PERK overexpression plasmid. Means ± SEM for six replicates are shown. *p < 0.05 compared to the control plasmid group with the Student’s t-test (C). Levels of RMRP were determined with Northern blotting. 28S and 18S are shown as control bands. The original gel images of Northern blotting are shown in Supplemental Fig. S4 (D).
