Figure 4
Silencing of RMRP leads to apoptosis in Huh7 and HLE cells. Huh7 and HLE cells were transfected with control siRNA, RMRP siRNA, or RMRP RNA for 24 h. Fold changes in RMRP were measured with RT-PCR. Means ± SEM for six replicates are shown. *p < 0.05 compared to the control siRNA group with the Student’s t-test (A). Apoptotic effect of RMRP knockdown. Apoptotic cells were positive for annexin V, and necrotic cells were positive for 7-amino-actinomycin D (B). Control siRNA and RMRP RNA were transfected at 50 pmol/L. Huh7 and HLE cells were treated with DMSO, tunicamycin, or tunicamycin + RMRP RNA (C). TUNEL staining of Huh7 and HLE cells. Cells transfected with control siRNA, RMRP siRNA1, and RMRP siRNA2 are shown. Scale bar represents 100 μm (D). Rates of apoptotic cells according to TUNEL staining. Apoptotic cells were evaluated with ImageJ software (National Institutes of Health, Bethesda, MD, USA). Means ± SEM for five replicates are shown. **p < 0.01 and *p < 0.05 compared to the control siRNA group with the Student’s t-test (E).
