Figure 2
From: Transcriptome analysis of human dermal fibroblasts following red light phototherapy
Transcriptomic and pathway enrichment profile of HDFs. The most considerable change in gene expression occurs between 0 and 4 h. (A) The number of up (red) and down (blue) DEGs by time for the 320 J/cm2 and (B) 640 J/cm2 fluences. (C) Representative expression profile of MMP1. Dashed and solid lines represent the 320 and 640 J/cm2 fluences, respectively. Blue lines are control samples, and red lines are RL treated samples. (D) Volcano plots of DEGs following irradiation with 320 J/cm2 and(E) 640 J/cm2 fluences. Red dots are DEGs with FDR-adjusted p values < 0.05 and fold change > 2 or < 0.5. Grey dots have FDR-adjusted p values > 0.05 or fold change between 2 and 0.5. DESeq2 was used to calculate DEGs for 320 J/cm2 and 640 J/cm2 RL treated samples. The number of DEGs was calculated by averaging the results of all four donor samples. Differential expression analysis was performed for each time point and the p-values were corrected for multiple testing using the FDR method. (F) Enriched GO pathways of interest, in 640 J/cm2 RL, all timepoints. (G) Co-regulatory networks of top 25 TF predicted to regulate differential expression in 640 J/cm2 RL treated HDFs and pooled time points.
