Figure 2
From: DOT1L inhibitors block abnormal self-renewal induced by cohesin loss
DOT1L inhibition rescues increased self-renewal phenotype of Rad21 knockdown. (a) Scheme of experimental design. Hematopoietic stem and progenitor cells (HSPCs) are isolated from the bone marrow of mice and infected with lentiviral shRNAs (a pLKO empty vector (grey) or one of two Rad21 shRNAs (red or blue), then the serial replating assay is performed. (b) Results of serial replating assay, displayed as number of colonies formed (CFUs) per 1000 cells plated for empty vector (black), Rad21 shRNA #1 (blue) and Rad21 shRNA #2 (red) treated with DMSO (solid) or DOT1L inhibitor EPZ4777 (forward slash) or EPZ5676 (backward slash). (c) Results of serial replating assay, displayed as fold expansion. B/C) n ≥ 2 per sample. Error bars represent the standard error of the mean. Statistical significance between individual data points determined by the Student’s T-test (two-tailed, unpaired). #p < 0.1, *p < 0.05, **p < 0.01, ***p < 0.001.
