Figure 5

Brain and body weights and brain myelin changes in Hom and Het mice. (A) Summary of brain and body weights in 6-month-old Hom and Het mice. n = 6 Hom, 7 Het. (B) Representative MRI images showing corpus callosum density between Hom and Het mice. Note extensive distribution of the myelin change in Hom mouse. Each panel represents an individual animal at one time point. (C) Scatter plot shows MRI density in corpus callosum. n = 5 Hom, 5 Het. (D) Nitroblue stained representative images showing difference in myelin staining intensity in corpus callosum of 6-month-old Hom and Het mice. Note relatively pale blue intensity in the corpus callosum of a Hom mouse as compared to the Het mouse. Yellow (pseudo) color demonstrates sparse myelin content in corpus callosum of a Hom mouse when compared to the intense red representing dense myelin in corpus callosum of a Het mouse. Processed images annotated to include corpus callosum for signal quantitation using HALO2.2% area algorithm. Nitroblue stain. (4×). (E) Scatter plot shows % myelin positive area when compared to the Het mice. Nitroblue stained sections analyzed using HALO2.2% area algorithm. n = 6 Hom, 7 Het. The graphical data for (A, C, E) are represented as mean ± SD (****p < 0.0001, **p = 0.0065, *p = 0.0428).