Figure3
CD14 is upregulated in CD45+CD11b+ leukocytes in the embryonic stroma following LPS stimulation. (A) Histogram showing CD326 expression in primary adult or embryonic intestinal stromal cells, with intestinal epithelial cells as a positive control. Data representative of n = 3 independent cultures generated from 3 adult mice or 3 litters of 6 embryos each. (B,C) Flow cytometric analysis of CD45 and CD11b expression in adult or embryonic intestinal stromal cells. The percentage of live CD45+CD11b+ cells were compared. n = 3 biological replicates. t-test ***p < 0.001. (D) Histogram depicting CD14 expression in CD45-CD11b− or CD45+CD11b+ populations in adult or embryonic intestinal stromal cells treated with LPS or vehicle control. (E) CD14 mean fluorescence intensity (MFI) in CD45+CD11b+ gated adult or embryonic intestinal stromal cells treated with LPS or vehicle. n = 3 biological replicates. One way ANOVA with Tukey’s multiple test. ****p < 0.0001.
