Figure 2 | Scientific Reports

Figure 2

From: Transcriptional circuitry atlas of genetic diverse unstimulated murine and human macrophages define disparity in population-wide innate immunity

Figure 2

Unified and distinct gene expression patterns of unstimulated bone marrow-derived macrophage (BMDM) across diverse murine strains reveal significant diversified immune system response signal pathways to maintain basal hemostasis. (a) PCA visualization of the RNA-seq in five mice strains (C57, BALB, NOD, PWK, and SPRET) was labeled with distinct colors. The plot showing highly consistent RNA-seq clustered together for each mice strain. (b) Heatmap of 6000 most variable expressed genes out of 12,218 BMDM expressed genes in five mice strains based on k-means clustering along with significantly enriched biological processes. The clustered columns represent the expression in mice strains, whereas each row represents a gene (adjusted P-value < 0.05, expression colored based on TPM z-score). (c) CC Bicluster algorithm (BCCC) discovered 830 highly correlated genes in cluster1 and significantly enriched in immune systems biological process across five mice strains (colored based on TPM z-score). (d) The number of differential expressed genes (DEGs) maintaining basal homeostasis between different mouse strains (FDR = 0.05, FC ≥ |2|). Red represents up-regulated and the green represents down-regulated genes from one to another strain. C57 and BALB have the least number of DEGs.

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