Figure 4
Differential chromatin accessibility and Transcription factors (TFs) interaction in unstimulated macrophage to maintain basal homeostasis in diverse murine strains. (a) Heatmap of accessible chromatin peak genes clustered by k-mean clustering and annotated with enriched biological processes in five mice strains. (b) Distribution of peaks in the promoter and distal region in five murine strains. (c) Heatmap of 124 Transcription Factors (TFs) was expressed across five strains of mice in RNA-seq (TPM > 1). The TFs are grouped according to their WGCNA assigned modules. The clustered columns represent the expression in mice strains, whereas each row represents a TF. Data is row-mean normalized and row clustered using correlation distance and average linkage. (d) Gene regulatory network (GRN) components of expressed TFs, expressed and chromatin accessible genes (TPM > 10) in C57,BALB, NOD, PWK, and SPRET murine strains. (e) Network analysis of individual GRNs revealed differential interactions for the same TF in all five mice strains at unstimulated macrophage (correlation (r2 = 0.99) and P < 0.0001 when compared to C57 strain). (f) ChIP-Seq of cJun and interactions of Jun in GRN of C57 murine model identified more than 57% of target genes correctly.
