Figure 5
The five consequential GRNs modeled pan basal regulation atlas of unstimulated macrophage in the murine model. (a) The pan GRN with 6375 nodes (6279 genes and 96 TFs) and 468,291 edges (interactions) across five mice strains. (b) Venn diagram representing the expression of unique and shared 96 TFs in five strains of mice. 57 TFs are expressed in all strains. (c) Heatmap of 96 Transcription Factors (TFs) present in pan GRN expressed across five strains of mice. The clustered columns represent TF expression, whereas each row represents different mice strain using correlation distance and average linkage. (Red: TPM > 10, White: TPM < 10). (d) Gene and pathway ontology identification of pan GRNs nodes by ClueGO. Some of the significantly enriched ontology terms are immune system, protein metabolism, signal transduction, transport, cell cycle, GPCR signaling, and RNA Metabolism (P ≤ 0.001) (Supplementary Figure S2). (e) Pan immune system GRN (5203 edges with 837 nodes). There are eight TFs (Stat3, Jun, Stat6, Rela, Irf9, Irf7, Irf3, and Atf1) involved in pan immune GRN. The bigger size of the node denotes Hub50 genes from the co-expression network analysis.
