Figure 7

Distinct TFs and GRNs in unstimulated macrophage of diverse genetically defined murine strains to maintain basal homeostasis. (a) Heatmap of 13 TFs expressed individually in each strain of mice (Average TPM ≥ 10). Total and unique interactions (genes with average TPM ≥ 10) are mentioned correspondingly. (b) Hgma2 and Ahctf1 specific C57 GRN (47 nodes, 72 edges). This GRN is involved in DNA damage and cell cycle. (c) Pou2f2, Sp100, Sp110, and Foxp1 specific NOD GRN (93 nodes, 273 edges). This GRN is involved in negative regulation of muscle cell differentiation, interferon signaling and mature B cell differentiation. (d) Runx1, Atf1, Zfp691, and Tcf12 specific PWK GRN (112 nodes, 434 edges). This GRN is involved in RUNX1 regulation of HSCs differentiation, signaling by NTRKs and transcription. (e) Egr1, Foxk1, and Egr3 specific SPRET GRN (221 nodes, 637 edges). This GRN is involved in interleukin-1 beta production, UCH proteinases and cell migration. There is no TF expressed only in BALB mice. Triangles are TFs and circles are expressed chromatin accessible genes. The gene and pathway ontology of GRNs nodes was done by ClueGO (P < 0.05).