Figure 6 | Scientific Reports

Figure 6

From: Selection and validation reference genes for qRT-PCR normalization in different cultivars during fruit ripening and softening of peach (Prunus persica)

Figure 6

Relative expression level of PpACS1 gene in ‘Hakuho’ peach cultivar from S1 to S4 stage. (a) Genes were normalized to ACTIN, TUB-B, TUB-A, RPIIand TEF2, individually and in combination, in ‘Hakuho’ peach cultivar. (b) Genes were normalized to TUB-A, TUB-B, TBP, RPIIand TEF2, individually and in combination, in S1. (c) Genes were normalized to UBC, HIS, ACTIN, TUB-A, EF-1α, RPII and TEF2, individually and in combination, in S2. (d) Genes were normalized to ACTIN, EF-1α, GAPDH, UBC, HIS, RPII and TEF2, individually and in combination, in S3. (e) Genes were normalized to ACTIN, TUB-B, HIS, eIF-4α, RPII and TEF2, individually and in combination, in S4. The statistical significance was determined by Duncan’s multiple comparison tests. Different letters indicate a significant difference (P < 0.05).

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