Figure 5
Change in protein expression level of ER stress marker after addition of TM to WT and RYR2V3599K neuronal cells. Representative images of (A) GRP78, (B) ATF6, (C) CHOP, (D) TAU/p-TAU, and the summarized data. Merged images with DAPI staining were also shown. p-TAU was expressed as the ratio to total TAU. DAN: dantrolene. Scale bars: 30 μm. Values for individual mice are plotted together with mean ± SEM. N = 19–32 cells from 3 to 6 mice. Parentheses indicate the number of mice. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA with post-hoc Tukey’s multiple comparison test in WT, unpaired t test in RYR2V3599K).
