Figure 2
Effects of secondary CPP on expression of fetuin-A mRNA in HepG2 cells. (A) HepG2 cells were treated with different concentrations (0, 25, 50, and 100 μg/mL) of synthetic secondary CPP for 24 h. (B) HepG2 cells were treated with 100 μg/mL secondary CPP for 0, 6, 12, 24 h. Total RNA was isolated from cells and fetuin-A/ahsg mRNA expression was determined using quantitative real-time PCR. Levels of fetuin-A mRNA were calculated using the comparative cycle threshold method, with 18S ribosomal RNA as the endogenous reference. Fetuin-A mRNA levels without secondary CPP (A) or at 0 h (B) were defined as 100% (control). Data are shown as mean ± SD from at least three independent experiments. *P < 0.05 vs. control.
