Figure 5
Effect of brefeldin A on secondary CPP-induced FM-fetuin-A. HepG2 cells were pre-treated for 30 min with or without 1 mg/mL brefeldin A and subsequently incubated in the absence or presence of the indicated concentrations of secondary CPP for 24 h. Immunoblots of cell lysates were probed with fetuin-A (upper panel) and α-tubulin (lower panel) antibodies. FM-fetuin-A levels were quantified using α-tubulin as an endogenous reference, with the fetuin-A level in the absence of CPP without brefeldin A defined as 100% (control). Data are shown as mean ± SD from at least three independent experiments. *P < 0.05 vs. control. Full-length blots/gels are presented in Supplementary Fig. X5 (full-size).
