Figure 6
Effect of BAPTA-AM on secondary CPP-induced FM-fetuin-A. HepG2 cells were cultured with 100 μg/mL secondary CPP in the absence or presence of 500 μg/mL of BAPTA-AM for 24 h. Immunoblots of cell lysates were probed with fetuin-A (upper panel) and GAPDH (lower panel) antibodies. FM-fetuin-A levels were quantified using GAPDH as an endogenous reference, with the fetuin-A level in the absence of CPP without BAPTA-AM as 100% (control). Data are shown as mean ± SD from at least three independent experiments. *P < 0.05 vs. control. Full-length blots/gels are presented in Supplementary Fig. X6 (full size).
