Figure 5
Milk EVs increase the number of proliferating Ki67 + cells in intestinal crypts in malnutrition. Number of proliferating cells in: controls i.e., sham-treated mice fed 18% protein diet; sham-treated mice fed 1% protein diet; and milk EV-treated mice fed 1% protein diet as represented by: (a) Immunofluorescent staining for proliferation marker Ki67 (red) in the jejunum and in the ileum with DAPI counterstaining of nuclei in blue; 40 × magnification (Scale bar, 25 µm) (b) Average count of Ki67 + cells per crypt in the jejunum and in the ileum. Each column represents mean ± standard error of mean (SEM) (****P < 0.0001, * P < 0.05, n.s = not significant, n = 5/group). Graphs and statistical tests were done using GraphPad Prism version 6.0.0 for Windows (www.graphpad.com).
