Table 1 Real-time PCR primer sequences.

From: Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition

Gene

Forward and Reverse primer sequence

Bmi1

F: CCAATGAAGACCGAGGAGAA

R: TTTCCGATCCAATCTGCTCT

Cldn3

F: ACCAACTGCGTACAAGACGAC

R: CGGGCACCAACGGGTTATAG

Lgr5

F: CGAGCCTTACAGAGCCTGATACC

R: TTGCCGTCGTCTTTATTCCATTGG

Rpl13a

F: TCCCTCCACCCTATGACAAG

R: GTCACTGCCTGGTACTTCC

Zo-1

F: GGAGATGTTTATGCGGACGG

R: CCATTGCTGTGCTCTTAGCG

Occludin

F: CACTGCACCCTGAGAAGCAT

R: CGAGCCTCCTTAGCTCGTAG

  1. Bmi1 B cell-specific Moloney murine leukemia virus integration site 1, Lgr5 leucine-rich repeat-containing G-protein coupled receptor 5, Rpl13a Ribosomal protein L13A, Zo-1 Zonulin-1.
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