Figure 2 | Scientific Reports

Figure 2

From: Vitamin D receptor upregulates lncRNA TOPORS-AS1 which inhibits the Wnt/β-catenin pathway and associates with favorable prognosis of ovarian cancer

Figure 2

Inhibition of ovarian cancer cell proliferation, invasion, migration, and colony formation by TOPORS-AS1. (A) RT-qPCR results showing TOPORS-AS1 expression in 6 ovarian cancer cell lines. (B,C) RT-qPCR results showing increased TOPORS-AS1 expression in IGROV1 and SKOV3 after TOPORS-AS1 transfection. (D,E) Cell proliferation assay showing slower proliferation of IGROV1 and SKOV3 after TOPORS-AS1 transfection. (F,G) Cell transwell migration assay showing reduced cell migration in IGROV1 and SKOV3 after TOPORS-AS1 transfection. (H,I) Cell transwell invasion assay showing reduced cell invasion in IGROV1 and SKOV3 after TOPORS-AS1 transfection. (J,K) Colony formation assay showing fewer colonies formed in IGROV1 and SKOV3 after TOPORS-AS1 transfection. (L,M) RT-qPCR results showing reduced TOPORS-AS1 expression after si-TOPORS-AS1 knockdown in IGROV1 and SKOV3 transfected with the TOPORS-AS1 plasmid. (N,O) Cell proliferation assay showing increased cell proliferation after si-TOPORS-AS1 knockdown in IGROV1 and SKOV3 transfected with the TOPORS-AS1 plasmid. (P,Q) Cell transwell migration assay showing increased cell migration after si-TOPORS-AS1 knockdown in IGROV1 and SKOV3 transfected with the TOPORS-AS1 plasmid. (R,S) Cell transwell invasion assay showing increased cell invasion after si-TOPORS-AS1 knockdown in IGROV1 and SKOV3 transfected with the TOPORS-AS1 plasmid. ***P < 0.0001; **P < 0.001.

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