Figure 4 | Scientific Reports

Figure 4

From: Vitamin D receptor upregulates lncRNA TOPORS-AS1 which inhibits the Wnt/β-catenin pathway and associates with favorable prognosis of ovarian cancer

Figure 4

Upregulation of TOPORS-AS1 expression by VDR and its effect on tumor cell proliferation, migration, and invasion. (A) PROMO prediction of a VDR binding site in the TOPORS-AS1 promoter. (B) Western blot results showing increased VDR expression in 293 T after transfecting the cells with a pCMV_VDR plasmid. (C) Luciferase reporter assay results showing increased luciferase activity in 293 T cells co-transfected with the VDR plasmid (pCMV_VDR) and luciferase reporter gene linked to TOPORS-AS1-wt (wild type) and no increase in TOPORS-AS1-mut (mutated). (D) Chromatin immunoprecipitation (ChIP) results suggesting VDR binding to the TOPORS-AS1 promoter. (E) Western blot results showing increased VDR and decreased β-catenin in IGROV1 and SKOV3 after VDR transfection. (F,G) RT-qPCR results showing increased TOPORS-AS1 expression in IGROV1 and SKOV3 after VDR transfection. (H,I) Cell proliferation assay showing slower IGROV1 and SKOV3 proliferation after VDR transfection. (J,K) Cell transwell migration assay showing fewer migrated IGROV1 and SKOV3 after VDR transfection. (L,M) Cell transwell invasion assay showing fewer invaded IGROV1and SKOV3 after VDR transfection. ***P < 0.0001; **P < 0.001.

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