Figure 2
From: Sex and Tamoxifen confound murine experimental studies in cardiovascular tissue engineering
TEVG Neotissue Formation. (A) H&E imaging of TEVGs at the 2-week time point. (B) Picro-Sirius Red images under polarized light, with close-up of TEVG wall (C). White spots represent PGA fibers; red/orange coloration represents mature thicker collagen fibers and yellow/green coloration represents thinner, immature collagen fibers, respectively. IHC quantifications of cellularity (D), tissue area (E), collagen area (F), collagen maturity (G), and Ki67 (H). RT-qPCR quantifications of TGF-β1 (I), TGF-β3 (J), and ratio of TGF-β1 to TGF-β3 (K). Scale bars = 0.5 mm (A), 200 µm (B) 20 µm (C). N = 15–20 for histology measures (D–G), and N = 6 for RT-qPCR measures (H–K), all compared using t-tests. *p < 0.05, **p < 0.01, ***p < 0.001.
