Figure 5
From: Sex and Tamoxifen confound murine experimental studies in cardiovascular tissue engineering
Tamoxifen Effects on Macrophage Response. (A) Representative imaging of CD68 staining, with giant cells labeled with black arrows. Total CD68 positive cells, including macrophages and giant cells quantified in (B) and giant cells alone quantified in (C). (D) Quantification of PGA polymer remaining in the wall of the TEVG 2 weeks after implantation. RT-qPCR quantification of pro-inflammatory NOS2 (E), anti-inflammatory Arg1 (F), and the ratio of the markers (G). RT-qPCR quantification of two markers of macrophage recruitment and function, CCL2 (H) and MMP9 (I). Scale bars = 200 µm. N = 15–20 for histology measures (B–D), and N = 6 for RT-qPCR measures (E–I), all compared using t-tests. *p < 0.05, **p < 0.01, ***p < 0.001.
