Figure 3

SLE patient cells do not survive in the presence of IFN-α. (A) PBMCs from SLE or healthy controls were isolated from blood and treated with IFN-α immediately after isolation and relative A3A expression quantified by qRT-PCR and normalized to the geometric mean of housekeeping genes RPL13A and GAPDH according to the Pfaffl method⁶⁵ for each sample. The fold change relative to the untreated sample is reported. (B) Untreated cells from the same donors as in A were stained for DSBs (γ-H2AX) after 4 h and 16 h in culture. (C) PBMCs from SLE or healthy controls were isolated from blood, left to rest overnight and only then treated with IFN-α. Relative A3A expression was quantified by qRT-PCR and normalized to the geometric mean of housekeeping genes RPL13A and GAPDH according to the Pfaffl method⁶⁵ for each sample. The fold-change in IFN-α-treated compared to non treated control 1 is reported. *p < 0.05; **p < 0.01; ***p < 0.001.