Figure 1 | Scientific Reports

Figure 1

From: Discovery of uncompetitive inhibitors of SapM that compromise intracellular survival of Mycobacterium tuberculosis

Figure 1

Primary screening of compounds. Enzymatic activity of SapM was assessed using the pNPP assay and expressed as percentage of specific activity (% SA). (A) Plot showing the effect of 1491 compounds at 100 µM on SapM activity (compounds ordered from left to right as LOPAC-1280, Enzo-BML-2834, LOPAC-Pfizer and in-house). Compounds that reduced SA by ≥ 50% (black dotted line) were selected. (B) Bar graph showing effect on SA of selected compounds from the primary screening (A) (bars in white) and re-tested compounds (bars in black). SA values of 0% overlap the x-axis. Percentage of SA is calculated relative to the pNPP DMSO control. Error bars indicate ± standard deviation of the mean (SD) of duplicates.

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