Figure 4
Effect of compound 1 on F. tularensis growth and intracellular burden. (A) Dose dependent efficacy of compound 1 on F. tularensis intracellular burden in MH-S macrophages at 24 h post infection. Bacterial burden was calculated from the average CFU/ml relative to DMSO control. Statistical significance was evaluated by one-way ANOVA (Dunnett’s test) compared to DMSO control (***p < 0.001). Error bars indicate ± SD of two independent experiments. (B) MH-S cells viability in percentage at 24 h post treatment with compound 1. Percentage of viability was calculated relative to the control (RPMI media only). Dashed black line indicates 70% viability. Error bars indicate ± SD of three independent experiments. (C) Effect of compound 1 on the acellular growth of F. tularensis monitored over 24 h using optical density (OD600). Cultures were treated with 40 µM of the inhibitor. Negative control is DMSO. Error bars indicate ± SD of triplicates.
