Figure 1 | Scientific Reports

Figure 1

From: Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer

Figure 1

Representative images of immune checkpoint molecules B7-H6 and PD-L1 localizations and abundance in breast cancer tissues using immunohistochemical staining. B7-H6 IHC analysis showed three localizations (a, Original magnification × 400): membranous tumor cells (a, green gated area), cytoplasmic tumor cells (a, blue gated area) and in the stroma area stained on immune infiltrated cells (TILs) (a, red gated area). Identically, PD-L1 IHC analysis showed three localizations (b, Original magnification × 400): membranous tumor cells (b, green gated area), cytoplasmic tumor cells (b, blue gated area) and in the stroma on TILs (b, red gated area). (c, Original magnification × 1000) B7-H6 and PD-L1 expression analysis by immunohistochemical staining at the level of breast cancer cells (IHC BCC). B7-H6 and PD-L1 IHC showed positive stained tumor cells at three different intensities; strong, moderate and weak. Positive controls for IHC staining of B7-H6 (Striated muscles tissue) and PD-L1 (Placenta tissue) respectively. (d) Original magnification × 400) B7-H6 and PD-L1 IHC showed positive stained immune cells in three different densities; Low, Medium and High B7-H6 + TILs or PD-L1 + TILs distribution in the stroma area are presented.

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