Table 3 Activities of the dye degrading enzymes after 9 h in consortium-BP (complete decolorization), Bacillus flexus TS8, Proteus mirabilis PMS, and Pseudomonas aeruginosa NCH.

From: Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes

Enzymes

Df

F-value

P-value

Bacillus flexus

Proteus mirabilis

Pseudomonas aeruginosa

Consortium-BP

Lignin peroxidase (intracellular)a

11

30.339

0.000*

0.849 ± 0.03

1.023 ± 0.03

0.514 ± 0.37

1.925* ± 0.012

Lignin peroxidase (extracellular)a

11

20.324

0.000*

0.072 ± 0.023

0.113 ± 0.029

0.153* ± 0.040

0.239** ± 0.000

Laccasea

11

7.113

0.012*

0.021 ± 0.01

0.018 ± 0.009

0.036 ± 0.008

0.056* ± 0.004

Tyrosinasea

11

11.685

0.003*

0.015 ± 0.006

0.010* ± 0.005

0.022 ± 0.005

0.035** ± 0.004

NADH-DCIP reductaseb

11

18.538

0.001*

11.84 ± 3.36

16.29* ± 4.07

20.84 ± 1.60

28.34*** ± 1.26

Azoreductasec

11

60.456

0.000*

1.97 ± 0.74

4.75 ± 0.56

6.63*** ± 0.91

9.98*** ± 0.75

  1. df: degrees of freedom, Values are mean of three experiments, SEM ( ±), significantly different from Bacillus flexus at *P < 0.05, **P < 0.01, and ***P < 0.001 and by one-way ANOVA with Tukey’s-HSD Comparisons Test.
  2. aU min−1 mg protein−1.
  3. bµg of DCIP reduced min−1 mg protein−1.
  4. cµM of methyl red reduced min−1 mg protein−1.
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