Figure 1 | Scientific Reports

Figure 1

From: Establishing an immunocompromised porcine model of human cancer for novel therapy development with pancreatic adenocarcinoma and irreversible electroporation

Figure 1

Generation of RAG2/IL2RG knockout pigs. (A) Schematic approach for RAG2/IL2RG knockout pig production. First, the sgRNA and Cas9 mRNA was synthesized via in vitro transcription. Second, an optimal concentration of sgRNA and Cas9 was injected into presumable zygotes. Third, the injected embryos were transferred into a surrogate gilt. At the end of gestation, piglets were born via hysterectomy and maintained in gnotobiotic isolators. (B) Summary of piglet genotypes in IL2RG and RAG2 gene. Six piglets were born and analyzed. During PCR analysis, RAG2 was not amplified from the Piglet #2 DNA sample, suggesting a large deletion. Biallelic genotype indicates two differently modified alleles. Homozygous indicates one type of modified alleles. (C) Genotype of Piglet 1 as a representation of small deletions or insertions introduced by the CRISPR/Cas9 system.

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