Figure 7

Primary human, SCID-like Panc01, and murine PDX tumors demonstrate similar conductivity increases following high voltage pulsed electric field. Ex vivo tissue samples directly from patients, RAG2/IL2RG animals, or NSG mice were sectioned and fit within a PDMS mold to impose a cylindrical shape factor. After sample preparation, individual samples were exposed to IRE pulses with varying electric field magnitudes. (A) Summary of initial conductivities calculated from normal and cancerous SCID-like (black), human (red), and murine PDX (gray) tissue. (B) The conductivity for each tissue type increased with the applied electric field. (C) Percent increase in tissue conductivity at varying fields was determined from the sample-specific pre-pulse values. (D) The adjusted conductivity is calculated from the percent difference values; here, the conductivities are normalized to the average initial tissue conductivity and adjusted based on the percent change.