Figure 1
From: Identification of critical amino acid residues in the regulatory N-terminal domain of PMEL
Proteolytic maturation of PMEL alanine-scanning mutants. Western blot analysis of SDS-lysed total membranes derived from Mel220 cells stably expressing PMEL alanine-scanning mutants. (A) PMEL-specific antibodies Pep13h and EPR4864 recognize the PMEL C-terminus. Note that P1 represents the ER form of the protein, while Mβ is generated through proteolytic cleavage in secretory compartments after PMEL has been released from the ER. The Mβ:P1 ratio is a measurement of the efficiency with which PMEL exits the ER, which past studies have shown is a good indicator for how well PMEL is folded. (B) Antibody HMB45 recognizes the sialylated fibril-associated fragments MαC and RPT. (C) Antibody I51 recognizes the fibril-forming CAF. Horizontal dashed lines separate different exposures of the same blot.
