Figure 5 | Scientific Reports

Figure 5

From: Enhanced anti-cancer activity of andrographis with oligomeric proanthocyanidins through activation of metabolic and ferroptosis pathways in colorectal cancer

Figure 5

Andrographis suppresses tumor growth in a xenograft animal model. (A) Schematic for the establishment of HCT116 cell line-derived xenograft in athymic mice, and the treatment schedule of andrographis and OPCs. (B) Representative image of xenograft tumor in each treatment group. (C) Tumor volume alterations in each treatment group. (D) Representative images of harvested tumors in each treatment group. (E) Bar graphs of harvested tumor weight in each treatment group. (F) Bar graphs showing mRNA expression of ferroptosis-related genes (HMOX1, GCLC and GCLM) of each treatment group in xenograft tumors. Statistical Significance is as follows: *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by one-way ANOVA test. (G) Alterations in protein expression levels of HMOX1, GCLC and GCLM following andrographis, OPCs, and combination treatment in tumor xenografts. Beta-actin was used as a loading control. Full-length blots are presented in Supplementary Fig. S3.

Back to article page