Figure 4

Migration and MMPs activities. Representative brightfield images of DHSF-BR16 and MCF-7 spheroid migration process, at T0, T72 and T144 h (intermediate time images not shown). (A) The graph shows the evaluation means of the spheroid diameters at the selected times. Data are the mean ± ES of at least three measurements. Images were obtained by using an inverted Leitz microscope , equipped with a 6MP Digital camera (CCD vision sensor, square pixels of 4.4 μm side length, 1600 × 1200 pixel resolution, 8-bit grey level) ). In (B) (MMP2) and (C) (MMP9), isoforms under basal conditions and FBS stimulation.