Figure 4
Effect of zoledronic acid on the periodontal ligament in the experimental groups Naive, Saline, ZA (0.2) and ZA (1.0). (A) Cross section of the root DV in HE staining (B) Mallory staining, × 400 magnitude, the bars indicate 50 µm (C) staining with HE and (D) picrosirius under polarized light. AB bone alevolar, D dentin, PDL periodontal ligament, P pulp. (E) Histomorphometry of the periodontal ligament area with analysis in relation to the total area of the PDL. Bars represent mean ± SEM of (n = 6/group). (*) indicates difference when compared to the Naive group. (#) indicates difference when compared to the Salina group. (§) indicates difference when compared to the ZA group (0.2) (ANOVA p < 0.001, Levene p = 0.043 and Tukey pos-hoc test). (F) Analysis of the mean by fields of the amount of blood vessels. Bars represent mean ± SEM of 6 animals per group. (*) indicates difference when compared to the Salina Group (kruskal Wallis test p = 0.015 and Dunn’s pos-hoc test). (G) Analysis of the blood vessel area on the tension side of the PDL in the experimental groups. Bars represent mean ± SEM of 6 animals per group. (*) indicates difference when compared to the Naive Group and (#) indicates difference when compared to the saline group (kruskal Wallis test p < 0.001 and Dunn’s pos-hoc test). (H, I) Histopathological analysis of the type I / type III collagen fibers ratio. Kruskal–Wallis test followed by Dunn’s post-test.
