Figure 1

Regulation of TSC1 by miR-130a. (a) The Western blot analysis shows the effect of overexpression of miR-92a and miR-130a on the level of TSC1 in SCC084 cells. Cells were transfected with 4 μg of empty vector pcDNA3-EGFP or constructs harboring different miRNAs cloned in this vector. Note, the reduced level of TSC1 in cells transfected with pmiR-130a (n = 2). (b) Dose-dependent reduction in the level of TSC1 at transcript and protein levels, following overexpression of miR-130a in SCC084 cells (n = 2). RT-qPCR data for TSC1 and miR-130a expression are an average of 2 technical replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001; and ns, non-significant (full-length blots are presented in Supplementary Figure S5).